The Institut Clinique de la Souris (ICS)
has developed a specific CreERT2 resource that is now available to the scientific community.
In this database you will find a standardized characterization of different Cre
lines driving Cre or Cre-ERT2 in various tissues and organs. We wish you to find
a Cre driver of interest for your research programs. We expect that it will
help to speed up your research program and the understanding of gene function
using temporal and spatial conditional mutagenesis.
The mouse lines described here are available for academic users under MTA
agreement and distribution fees. The fees for these animals are calculated on
cost recovery basis and are used to recover animal husbandry and stock
replacement costs. (Fees do not include the costs for shipping the material from
the ICS facility to the recipient's institution.)
Distribution fees
Live Mice (6-7 weeks of age, 2-3 Breeding pairs): 2400 €
Cryopreserved embryos (about 50 embryos in 2 straws): 1100 €
In most of the case only heterozygous carrier is being used for archiving and
thus the distributed materiel may contain carrier (mutant or transgenic) and
control (not mutant or not transgenic) embryos or gametes.
ICS offers additional breeding services to e.g. generate cohorts of homozygous
mutants. A quotation for such a service is available upon request.
Please note that all the lines are not specifically maintained for distribution.
We thus cannot guaranty immediate shipment of live animals you requested.
Once a request of interest is received, you will be contacted by email within a
week to give you an up-to date availability of the requested line.
More details on the restrictions, on PI and on the distribution is available
upon request.
Summary
The table below displays the number of characterized lines.
generated lines
39
lines tested for cre expression (RT-qPCR screening)
36
lines tested for cre expression (RT-qPCR extensive analysis - cre and endogenous gene)
24
evaluation of cre activity on colorimetric floxed reporter mice
15
lines cryo-preserved
11
What is a creERT2 mouse?
The fusion protein Cre ERT2 mediates spatio-temporal control of somatic mutagenesis.
It has become in the last years the state-of the art technology for in vivo study of gene
expression and gene function as deletion of a gene can be specifically targeted in a
tissue-specific manner.
Principle ->
Why developing this resource?
Generation of conditional knock-out mice has become classical.
Also in less than two years time, the three main international mouse mutagenesis programs
(EUCOMM for Europe, NorCOMM for the Canada and KOMP for the US) will terminate the
conditional targeted of most of the mouse gene. All these ES cells will be available to be
driven into mice.
A need for a significant number of Cre and CreERT2 new lines for cell and/or tissue
specific deletion is striking. The ICS CreERT2 zoo proposes new inducible Cre
lines to the scientific community, specific for various biological systems.
These lines have been selected by RT-qPCR (Cre mRNA) and look promising for their capacity to
delete floxed genomic fragment after Tamoxifen induction. The level of characterization of
these lines will be updated regularly in our database.
Most of the lines described here are still under further characterization and the data shown
here can be subjected to modification without specific notification.
Further information about the resource
The majority of the lines had been produced by pronuclear injection of BAC constructs
in FVB/N background and some projects in C57BL/6N background. Since April 2010 all the lines
are backcrossed in C57BL/6N background.
Data on the Cre mouse lines will continue to be expanded as soon as they are acquired.
About ICS
The Institut Clinique de la Souris is a technology platform that provides a comprehensive
set of specialized mouse services to scientists from academia and industry. The ICS combines
the capacity to generate mutant mice on a large scale with a high-throughput and comprehensive
phenotypic analysis of mice. The ICS phenotyping platforms are adapted for the study of
genetically engineered mouse models (GEMMs) and genetic reference populations (GRPs).
The services of the ICS will ultimately help the scientific community to use the mouse to
develop a complete functional annotation of the human genome and to employ this to better
understand human diseases and their underlying physiological and pathological basis.
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