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Departments

Follow ICS

Growth rate

Purpose

This test evaluates body-mass progression with age. The frequency of body-weight measurement depends of client's requirements.

Strain background references

Body weight progression under CD between different strains of female mice

Body weight progression under CD between different strains of male mice

Champy MF, Selloum M, Zeitler V, Caradec C, Jung B, Rousseau S, Pouilly L, Sorg T, Auwerx J. Genetic background determines metabolic phenotypes in the mouse. Mamm Genome. 2008 May;19(5):318-31. Epub 2008 Apr 5.

Recommendations 

8 mice per group are recommended for reliable data analysis.

Dysmorphology screen

Purpose

The test is performed to examine morphological abnormalities with respect to general physical appearance and body shape on mice: weight, length, and obvious dysmorphologies in the physical appearance, i.e. tail kinks, shape of ears, eyes, head, teethes, limbs, number and shape of digit, irregularities and variation in coat color, hair distribution and development, irregularities in the genitals (Fuchs H, et al., 2000).

Strain background references

Body temperature in different mice strains

Recommendations

8 mice per group are recommended for reliable data analysis of temperature and body-weight parameters.

Body composition

Purpose

The test gives precise analyses of the body composition  with respect to fat content, lean tissues and free body fluids in a mouse.

Strain background references

Change in body composition induced by 10 weeks of high fat diet (HFD) feeding in male mice from different strains: fat tissue

Change in body composition induced by 10 weeks of high fat diet (HFD) feeding in male mice from different strains: body weight

Change in body composition induced by 10 weeks of high fat diet (HFD) feeding in male mice from different strains: lean tissue

Equipment

Body composition is evaluated by Quantitative Nuclear Magnetic Resonance (qNMR) using Minispec+ analyzer (Bruker BioSpin S.A.S., Wissembourg, France)

Recommendations

8 mice per group are recommended for reliable data analysis.

Food intake

Purpose

Food consumption is measured by manually weighing remnants of food in the feeders. The test gives precise or estimate evaluation of energy intake in mice depending of whether the animals are singly or socially housed. Food consumption (and water consumption) may also be assessed more accurately in metabolic cages or in polymodal indirect calorimetry monitoring system (see below).

Strain background references

Equipment (usage optional)

Metabolic and diuresis cages (Line 3, Techniplast).

Recommendations

8 mice per group are recommended for reliable data analysis.

Energy expenditure by indirect calorimetry

Purpose

Calorimetry cages allow the simultaneous measurement of energy expenditure, ambulatory activity as well as food and water intake. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. It also monitors CO2 production, thus the respiratory exchange ratio (RER) and finally heat production can be calculated. Infra-red frames are integrated to the system in order to measure ambulatory activity. A food intake monitoring system is also integrated for the measurement of feeding/drinking behaviors.

Experiments can be performed from 24h to 5 consecutive days.

The test can be conducted at different ambient temperature (4°C to 39 °C) and the cages allow  one to performing food and drink restriction procedures as well as food or drink preference assays.

Strain background references

Equipment


Home cage system (12 cages) (TSE LabMaster systems GmhB, Bad Homburg, Germany)

Recommendations

  • 8 mice per group are recommended for reliable data analysis.
  • Energy expenditure and associated physiological parameters will change with respect to age, sex and strain differences.
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