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The Genetic Engineering and Model Validation department is in charge of the generation of customized and ready-to-use genetically modified mouse.

Since its creation in 2002, more than 1300 mouse models were done for our customers: academic labs, European Union or big pharmaceutical groups.

We offer quality custom generation of a wide range of mouse models developed by microinjection of Sv129/Pas or C57BL/6N ES cells into blastocysts or by DNA pronuclear injection. 

We also focus our efforts on reducing the timelines by developing new techniques and standardizing our process. CRISPR-Cas9 is a rapid and cost effective technology to generate mouse and rat mutant models. Now we can offer the generation of knock-out models though direct injection into fertilized mouse oocytes of CRISPR sgRNA and Cas9 mRNA. This approach allows to obtain models in shorter timelines and with reduced costs compared to gene targeting by homologous recombination in ES cells. Finally, CRISPR/Cas9 genome editing opens a new area in rat genetics, and we are now able to provide researchers with rat models.To secure your project, we provide quality control procedures and develop a quality management system / assurance.

Each model can be validated at the DNA (ddPCR, Southern Blot), RNA (Northern Blot) or protein level (Immunohistochemistry, Western Blot) by our genotyping and gene expression services

Available models


The development of a genetically modified mouse line by homologous recombination includes:

  • The design of a strategy for the modification of the targeted gene
  • The construction of the target vector
  • The electroporation in 129 S2/SvPas ES or in C57BL/6N cells
  • The screening of up to a maximum of 700 ES colonies and the confirmation of ES positive clones if needed
  • The development of chimeric mice
  • The establishment of the genetically modified mouse line by breeding with the chimeric mice (Germ Line Transmission (GLT) of the mutation)
  • The in vivo excision of the selection marker by breeding
  • The delivery of at least 2 heterozygous mice for the requested type of mutation (cKO, KI, ...)
  • The delivery of a technical report that detailed the main results obtained in each step and includes a genotyping protocol
  • On request: validation of the functionality of the genetic modifications introduced in vivo

The development of a transgenic mouse by micro-injection of a DNA construction into the pronucleus of fertilized eggs (PNI transgenesis) includes:

  • The design of a strategy of the vector development for the transgenic mouse model
  • The construction of the target vector
  • The micro-injection of the vector into a maximum of 300 C57BL/6N mice eggs (other backgrounds on request)
  • The genotyping of the founders and their delivery
  • On request: generation of G1 animals and qRT-PCR evaluation of expression of each line

These services are 100% customized: each step can be performed or not in our institute.

Webinars CRISPR/Cas9 in rodents

PHENOMIN-ICS presented 2 webinars on CRISPR/Cas 9 in rodents that were hosted by our partner Charles River.

We are pleased to remind they are now avaibale as replay !

-CRISPR/Cas9 in Rodents Animals: The good, The bad and the ugly: March 30th  -  CRISPR/Cas9 has revolutionized the creation of complex mouse and rat models. Genome editing opportunities can seem infinite, but there are parameters that must be considered. 


-Modelling Human Disease in Rodents with CRISPR/Cas9 Genome Editing : June 27th - Why do the results discovered using genetically engineered mice sometimes fail to translate to humans? This webinar will discuss the promise CRISPR/Cas9 has for rodent studies along with strategies for developing better models to understand and treat human disease.



July 4, 2017


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