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Glucose tolerance test

Purpose

The glucose tolerance test is commonly used to assess the ability of standardized glucose bolus to induce hyperglycaemia. The glucose is either administrated by oral gavage or by intra peritoneal injection. The blood glucose level is measured at different time points after administration.

Strain background references

OGTT after 12 weeks upon HFD

Glucose AUC

Recommendations

8 mice per group are recommended for reliable data analysis.

Insulin sensitivity test

Purpose

The insulin sensitivity test is commonly used to assess the ability of an intraperitoneal insulin load (0.5 to 1 U.I/kg) to induce hypoglycemia. Insulin is administrated by intra-peritoneal injection and blood glucose is measured at different time points after administration. 

Strain background references

IPIST after 14 weeks upon high fat diet upon different strains

Recommendations

8 mice per group are recommended for reliable data analysis.

Hyperinsulinemic euglycaemic clamp

Purpose

Alterations in insulin sensitivity (resistance or hypersensitivity) can be revealed on the basis of an insulin sensitivity test. However a more reliable quantitative analysis is performed using the Euglycaemic Hyperinsulinemic clamp technique. In this procedure insulin is administrated to raise plasma insulin concentration while glucose is infused to maintain euglycaemia. The glucose infusion rate needed to maintain euglycaemia is a reflection of insulin action in the whole body when both glycemia and hyperinsulinemia are set at constant. A femoral vein catheter is placed under anaesthesia 3 days prior to the experiment. The test is conducted on 5h-fasted vigil and not restrained mice.

Strain background references

Hyperinsulinemic euglycaemic clamp in C57Bl/6J mice fed standard diet (CD) and high fat diet (HFD)

Recommendations

8 mice per group are recommended for reliable data analysis.

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