ICS line text

Departments

Follow ICS

Complete blood count (CBC)

Purpose

Hematological analysis of blood determines blood cell counts (leukocytes, erythrocytes and platelets) and gives hemoglobin levels. Additional hematological parameters (hematocrit, mean cell volume, mean corpuscular hemoglobin, mean cell hemoglobin concentration) can be calculated. The different white blood cells (WBC) lines: neutrophils, lymphocytes, monocytes, eosinophils, and basophils are also identified and counted.

Strain background reference

Champy MF, Selloum M, Zeitler V, Caradec C, Jung B, Rousseau S, Pouilly L, Sorg T, Auwerx J. 
Genetic background determines metabolic phenotypes in the mouse. 
Mamm Genome. 2008 May;19(5):318-31. Epub 2008 Apr 5.

Equipment

CBC is performed on an Advia 120 (Siemens AG, Erlangen, Germany) with  Veterinary software.

Recommendations

Within the same cohort, approximately 9 mice per group are recommended for reliable data analysis.

Coagulation

Purpose

Determination of prothrombine time, activated partial thromboplastin time and fibrinogen activity.

Equipment

ST4 semi automated coagulometer (Diagnostiqua Stago, Gennevilliers, France).

Recommendations

It is recommended to collect blood by intra-cardiac puncture.

Within the same cohort, approximately 9 mice per group are recommended for reliable data analysis.

Blood hematology in different mouse strains at the age of 3 months (Platelets)

Blood hematology in different mouse strains at the age of 3 months (Leukocytes)

Blood hematology in different mouse strains at the age of 3 months (Erythrocytes)

search icon