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Purpose

The study of the immune system allows checking immunological disorders (autoimmune diseases, hypersensitivities, immune deficiency,...) and pathogenic infection. A large panel of mouse cytokine analysis are available. Immunoglobulins (IgG subclasses, IgM, IgA and IgE) can be measured in plasma samples. Thymus and spleen cell lineage are determined by flow cytometry.

Several phenotypes may be identified using in vivo inflammatory disease models.

Tests

  • Immunoglobulins (IgG1, IgG2a, IgG2b, IgG3, IgM, IgA and IgE) 
  • Cytokines (Eotaxin, G-CSF, GM-CSF, IFN-γ, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IP-10, KC, LIF, LIX, M-CSF, MCP-1, MIG, MIP-1α, MIP-1β, MIP-2, RANTES, TNF-α, VEGF)
  • FACS analysis 
    • Thymus: 
      • T cells of ab lineage: CD4/CD8/TCRab 
      • T cells of gd lineage: CD4/CD8/TCRgd 
      • NKT cells: NK1.1/CD3 
    •  Spleen: 
      • B cells: CD19/IgM/IgD/CD80 
      • B cells: CD19/IgM/CD2/CD23 
      • NK cells and NKT cells: DX5/NK1.1/CD3 
      • Macrophages and neutrophils: F4-80/Gr-1/Mac-1 
      • T cells: CD4/CD8/CD3/CD44 

Equipment

  • Cytokines and immunoglobulins are measured by immuno-analysis on a Multiplex analyser. 
  • FACS analysis are performed on a LSR II (BD diagnostics, Le pont de Claix, france).

Recommendations

Within the same cohorte, 9 mice per group are recommended for reliable data analysis on blood samples. At least, 6 mice per group are required for data analysis on tissue sample.

Upon request, certain immune challenges can be performed (ex. LPS,...)

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