Departments

Head

Marie Wattenhofer-Donzé

Staff

4 persons

Missions

The ES cells service is in charge of the different culture steps done to produce mutant ES lines by homologous or illegitimate recombinations. The service has also the capability to generate its own ES lines and had developed differentiation protocols as a tool for cellular phenotyping.

C57BL/6N, 129Sv/Pas and BALB/cN ES lines were established in our institute and validated for high germ line transmission efficiency.

Actvities

ES Culture Services
- Growing and freezing of ES cells with different backgrounds
- Electroporation
- Subcloning and amplification of targeted clones
- Karyotyping of mutated ES clones before microinjection into recipient blastocysts
Supplementary activities
- Generation of wild type or mutated mouse ES lines within different genetic backgrounds
- In vitro differentiation of ES lines