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Departments

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Head

Sylvie Jacquot

Staff

6 persons

Missions

This service is in charge of genotyping mice housed in the institute. The platform offers customized genotyping services for knock-out, knock-in, transgenic or any other genetically modified mouse strains. Different sample types can be processed, including tails and ear tags. All genotyping steps are covered: a complete design, optimization, verification of PCR amplification conditions for new or pre-existing strategies, genomic DNA extraction, PCR and/or qPCR reactions, analysis and sequencing for the gene of interest. This fully automated service allows a high-throughput process using the robotic (Tecan, Hamiton) workstations (DNA extraction, PCR, qPCR...) as well as Light cycler (Roch) for qPCR product analysis. All these technologies are driven by in-house databases insuring high quality of data at a large scale level.

Techniques

  • PCR, qPCR

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