The Resources technologies service is in charge of internal research and development programs. The main goal of this service is to create new mouse tools for the community.
In order to facilitate the use of the new mutant resources developed in the mouse, we have generated Cre and FlpO deleter mice on a pure inbred C57BL/6N background. The new transgenic constructs were designed to drive either the Cre or FlpO recombinase, fused to a specific fluorescent marker (respectively the eGFP or the eYFP) and were inserted by homologous recombination in the neutral Rosa26 locus. They allow a rapid, cost-effective and efficient identification of the carrier individuals through the co-expression of the fluorescent marker.
The main advantages of these deleters are:
Request the FlpO deleter: Gt(ROSA)26Sor<tm2(CAG-flpo,-EYFP)Ics (EM:05490)
Request the Cre deleter: Gt(ROSA)26Sor<tm1(ACTB-cre,-EGFP)Ics (EM:05488)