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Departments

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Missions

The Resources technologies service is in charge of internal research and development programs. The main goal of this service is to create new mouse tools for the community.

Resources

  • CreERT2 zoo : database of more than 50 cell or tissue specific promoters driven CreERT2 transgenic lines.
  • NR Zoo : database of the nuclear receptors and there co-factors developed as mouse conditional knock-out models.
  • MousePat : database providing comprehensive analysis of the Nuclear Receptors (NRs) expression in the adult mouse brain.

Techniques

  • qPCR, RT-qPCR
  • lacZ staining
  • in situ hybridization
  • Immunohistochemistry

New tools

Highly-efficient, fluorescent, locus directed Cre and FlpO deleter mice on a pure C57BL/6N genetic background

In order to facilitate the use of the new mutant resources developed in the mouse, we have generated Cre and FlpO deleter mice on a pure inbred C57BL/6N background. The new transgenic constructs were designed to drive either the Cre or FlpO recombinase, fused to a specific fluorescent marker (respectively the eGFP or the eYFP) and were inserted by homologous recombination in the neutral Rosa26 locus. They allow a rapid, cost-effective and efficient identification of the carrier individuals through the co-expression of the fluorescent marker.

The main advantages of these deleters are:

  • Preservation of a pure genetic background when used to remove specific selection cassette or to induce complete loss-of-function allele (pure inbred C57BL/6N background)
  • Insertion in the Rosa26 locus
  • Simplicity of genotype identification (fluorescence evaluation)
  • High and stable recombination efficiency (up to 100%)
  • Expression of cre and FlpO in developing oocytes irrespectively of the transmission of the Cre and FlpO transgenes

Publication

Request the FlpO deleter: Gt(ROSA)26Sor<tm2(CAG-flpo,-EYFP)Ics (EM:05490)

Request the Cre deleter: Gt(ROSA)26Sor<tm1(ACTB-cre,-EGFP)Ics (EM:05488)

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