This test is frequently used for screening of the potential antiepileptic drugs and is based on the excitotoxic properties of pentylene-tetrazol (PTZ). This substance acts as a non-competitive GABA-A receptor antagonist that reduces neuronal inhibition.
Do preclinical seizure models preselect certain adverse effects of antiepileptic drugs.
Epilepsy Res. 2002 Jun;50(1-2):33-40.
There is no dedicated equipment: observation and manual scoring
10 mice per group are recommended for reliable data analysis