ICS line text

Phenotyping

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Tail flick test

Purpose

The tail flick test measures a simple spinal reflex to a sudden painful thermal stimulus.
A photobeam is used to apply a heat stimulus to the tail, and the time to flick the tail out of the path of the light beam is measured.

Equipment

Tail flick equipment (Panlab, Barcelona, Spain)

Recommendations

10 mice per group are recommended for reliable data analysis.

Hot plate test

Purpose

The hot plate test is used for evaluation of response to acute thermal pain.
The mice are placed into a glass cylinder on a hot plate adjusted to 52°C and the latency of the pain reaction (licking, moving the paws, little leaps) is recorded. 

Equipment

Hot plate (Panlab, Barcelona, Spain).

Recommendations

10 mice per group are recommended for reliable data analysis.

Formalin pain

Purpose

Formalin tests allows evaluation of chronic inflammatory pain.

Equipment

There is no dedicated equipment: observation and manual scoring are used.

Recommendations

10 mice per group are recommended for reliable data analysis.

Shock threshold

Purpose

This procedure is used to assess sensitivity to electrical footshock, and is a critical control for  procedures requiring foot-shocks (i.e, aversive learning).

Equipment

Fear conditioning equipment (Coulbourn Instruments, Whitehall, USA)

Recommendations

10 mice per group are recommended for reliable data analysis.

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